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Article

Temperature-sensitive expression of all-Torpedo and Torpedo-rat hybrid AChR in mammalian muscle cells.

The Journal of cell biology - 1 May 1990

Paulson H L, Claudio T

Abstract excerpt

When the four subunits of the Torpedo californica nicotinic acetylcholine receptor (AChR) are expressed in mammalian fibroblasts, they properly assembly into alpha 2 beta gamma delta pentamers only at temperatures lower than 37 degrees C (Claudio, T., W. N. Green, D. S. Hartman, D. Hayden, H. L. Paulson, F. J. Sigworth, S. M. Sine, and A. Swedlund. 1987. Science (Wash. DC). 238:1688-1694). Experiments here with...

Topics

Animals
Antibodies, Monoclonal
Bungarotoxins
Cell Line
Macromolecular Substances
Muscles
Mutation
Precipitin Tests
Protein Conformation
Rats
Receptors, Cholinergic
Recombinant Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Temperature

Torpedo

Transfection

Source

Open in PubMed Open full text

Identifiers

PMID: 2335568
DOI: 10.1083/jcb.110.5.1705
PMCID: PMC2200171

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2335568-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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