Article
Expression and assembly of Torpedo californica (Na,K)ATPase alpha-subunit truncated at N-terminal end in Xenopus oocytes.
Biochemistry international - 1 Apr 1992
Mutoh Y, Noguchi S, Ohta T, Higashi K, Takasugi M, Kuroiwa A, Kawamura M
Abstract excerpt
cDNAs for mutant alpha-subunits of Torpedo californica (Na,K)ATPase variously truncated at the N-terminal end were constructed and transcribed in vitro. Each of the mRNAs thus synthesized was co-injected into Xenopus oocytes together with mRNA for wild-type beta-subunit. Truncation of the alpha-s...
Topics
- Animals
- Base Sequence
- DNA
- Female
- Gene Expression
- In Vitro Techniques
- Kinetics
- Molecular Sequence Data
- Mutation
- Oocytes
- Ouabain
- Protein Conformation
- RNA, Messenger
- Sodium-Potassium-Exchanging ATPase
- Torpedo
- Transcription, Genetic
- Xenopus