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Article

Structure and function in rhodopsin: a tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants.

Proceedings of the National Academy of Sciences of the United States of America - 15 Oct 2002

Reeves Philip J, Kim Jong-Myoung, Khorana H Gobind

Abstract excerpt

Tetracycline-inducible HEK293S stable cell lines have been prepared that express high levels (up to 10 mg/liter) of WT opsin and its mutants only in response to the addition of tetracycline and sodium butyrate. The cell lines were prepared by stable transfection of HEK293S-TetR cells with expression plasmids that contained the opsin gene downstream of a cytomegalovirus promoter containing tetO sequences as well...

Topics

Animals
Bioreactors
Cattle
Cell Line
Gene Expression
Glycosylation
Humans
Mutation
Recombinant Proteins
Rhodopsin
Rod Opsins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Tetracycline

Transfection

Tunicamycin

Source

Open in PubMed Open full text

Identifiers

PMID: 12370422
DOI: 10.1073/pnas.212519199
PMCID: PMC129687

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