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Article

Narrowing substrate specificity in a directly evolved enzyme: the A293D mutant of aspartate aminotransferase.

Biochemistry - 12 Oct 2004

Chow Margaret A, McElroy Kathryn E, Corbett Kevin D, Berger James M, Kirsch Jack F

Abstract excerpt

Several mutant Escherichia coli aspartate aminotransferases (eAATases) have been characterized in the attempt to evolve or rationally redesign the substrate specificity of eAATase into that of E. coli tyrosine aminotransferase (eTATase). These include HEX (designed), HEX + A293D (design followed by directed evolution), and SRHEPT (directed evolution). The A293D mutation realized from directed evolution of HEX is...

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Identifiers

PMID: 15461450
DOI: 10.1021/bi0487544

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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