Article
Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris.
BMC biotechnology - 10 May 2011
Nordén Kristina, Agemark Maria, Danielson Jonas Å H, Alexandersson Erik, Kjellbom Per, Johanson Urban
Abstract excerpt
BACKGROUND: When performing functional and structural studies, large quantities of pure protein are desired. Most membrane proteins are however not abundantly expressed in their native tissues, which in general rules out purification from natural sources. Heterologous expression, especially of eu...
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