Article
Role of the tertiary nucleotides in the interaction of yeast phenylalanine tRNA with its cognate synthetase.
Biochemistry - 13 Mar 1990
Sampson J R, DiRenzo A B, Behlen L S, Uhlenbeck O C
Abstract excerpt
In vitro transcription by T7 RNA polymerase was used to prepare 32 different mutations in the 21 nucleotides that participate in the 9 tertiary base pairs or triples of yeast tRNAPhe. The mutations were designed either to disrupt the tertiary interaction or to change the sequence without disrupting the structure by transplanting tertiary interactions present in other tRNAs. Steady-state aminoacylation kinetics...
Topics
- Amino Acyl-tRNA Synthetases
- Base Composition
- Base Sequence
- Hydrogen Bonding
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Phenylalanine-tRNA Ligase
- RNA, Transfer, Amino Acid-Specific