Article
Detection of coinherited Hb H-Constant Spring/Paksé disease and Hb E by capillary electrophoresis and high performance liquid chromatography.
Hemoglobin - 1 Jan 2013
Pornprasert Sakorn, Waneesorn Jarurin
Abstract excerpt
A capillary electrophoresis (CE) method has been proven to be superior to a high performance liquid chromatography (HPLC) method in the detection of Hb H-Constant Spring/Paksé [Hb H (β4) Hb CS or α142, Term→Gln, TAA>CAA (α2)]/Paksé [α142, Term→Tyr, TAA>TAT (α2)]. It also has the ability to quantify Hb Bart's (γ4). The aim of this study is to analyze the efficacy of the CE and HPLC in the detection of Hb H...
Topics
- Chromatography, High Pressure Liquid
- DNA Mutational Analysis
- Electrophoresis, Capillary
- Hemoglobin E
- Hemoglobin H
- Hemoglobinopathies
- Hemoglobins, Abnormal
- Humans
- Mutation
- Reproducibility of Results
- Sensitivity and Specificity
- Thailand
- Thalassemia