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Article

A rapid PCR-based method for the identification of ob mutant mice.

Obesity (Silver Spring, Md.) - 1 Feb 2009

Ellett Justin D, Evans Zachary P, Zhang Guojing, Chavin Kenneth D, Spyropoulos Demetri D

Abstract excerpt

With increasing incidence of obesity, there is greater demand for suitable research and therapeutic models. The ob/ob mouse model develops obesity by 5 weeks of age. Previously, a method using DNA purification, PCR, and restriction digestion of products was devised to identify mice bearing the ob allele. Here, we describe a direct PCR method that requires no DNA purification. Wild-type and ob-specific primers are...

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PMID: 18948969
DOI: 10.1038/oby.2008.443

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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