A novel and quick PCR-based method to genotype mice with a leptin receptor mutation (db/db mice).

Abstract excerpt

db/db mice is one of most widely used animal models in studying the cellular and molecular mechanisms of metabolic disorders, such as diabetes, hyperlipidemia, and obesity. The mice carry spontaneous point mutations in the gene encoding the leptin receptor, leading to leptin receptor inactivation. Since homozygous db/db mice are sterile, the maintenance of db/db mice requires breeding between heterozygous pairs,...

Topics

• Animals
• DNA Primers
• Genotype
• Genotyping Techniques
• Mice
• Point Mutation
• Polymerase Chain Reaction
• Receptors, Leptin
• Reproducibility of Results
• Sensitivity and Specificity