Article
Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lepob) mutation.
Obesity research - 1 May 1997
Chung W K, Chua S C, Lee G H, Leibel R L
Abstract excerpt
Three polymerase chain reaction-based assays for the mouse Lepob mutation are described: Dde I site created by the C-->T transversion characterizing Lepob enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence whic...
Topics
- Amino Acid Sequence
- Animals
- Electrophoresis
- Genotype
- Mice
- Mice, Obese
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length