Article
Genetic typing of the mouse ob mutation by PCR and restriction enzyme analysis.
Experimental animals - 1 Jan 1997
Hirasawa T, Ohara T, Makino S
Abstract excerpt
A genetic typing method for the mouse obese (ob) mutation by PCR and restriction fragment length polymorphism (RFLP) analysis was developed. Three genotypes (ob/ob, ob/+ and +/+) of the ob mouse are rapidly differentiated with this assay. Since only a small biopsy specimen (tail end) is necessary for the genotyping, the ob mouse at any age is typable and can be used in subsequent breeding or research. Obese...
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