Article
Genetic typing of the mouse ob mutation by PCR and restriction enzyme analysis.
Experimental animals - 1 Jan 1997
Hirasawa T, Ohara T, Makino S
Abstract excerpt
A genetic typing method for the mouse obese (ob) mutation by PCR and restriction fragment length polymorphism (RFLP) analysis was developed. Three genotypes (ob/ob, ob/+ and +/+) of the ob mouse are rapidly differentiated with this assay. Since only a small biopsy specimen (tail end) is necessary...
Topics
- Animals
- DNA Restriction Enzymes
- Genotype
- Mice
- Mice, Inbred C57BL
- Mice, Obese
- Mutation
- Obesity
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length