Article
Transfection of cDNA with G----T point mutation at the cleavage site of insulin receptors to COS 7 cells.
Biochemical and biophysical research communications - 30 Mar 1990
Kobayashi M, Sugibayashi M, Sasaoka T, Egawa K, Shigeta Y, Tamaki M, Nakamura E, Teraoka H
Abstract excerpt
To study whether the G----T point mutation of insulin proreceptors at the cleavage site which changed -Arg-Lys-Arg-Arg- to -Arg-Lys-Arg-Ser- caused unprocessed insulin receptors with decreased insulin binding affinity, we performed transfection of cDNA with the mutation in COS 7 cells and examined the expressed insulin receptors. After site-directed mutagenesis, an expression vector pGEM3SV was used to make a...
Topics
- Amino Acid Sequence
- Animals
- Cell Line
- DNA
- Gene Expression
- Genetic Vectors
- Humans
- Insulin Resistance
- Kinetics
- Molecular Sequence Data
- Mutation