Article
Characterization of unprocessed insulin proreceptors in COS 7 cells transfected with cDNA with Arg735----Ser735 point mutation at the cleavage site.
Metabolism: clinical and experimental - 1 Aug 1992
Sugibayashi M, Shigeta Y, Teraoka H, Kobayashi M
Abstract excerpt
We previously reported on patients with severe insulin resistance due to unprocessed insulin proreceptors. A structural change of the cleavage site from Arg-Lys-Arg-Arg to Arg-Lys-Arg-Ser due to G----T point mutation appeared to be the cause for failure to process the proreceptors. To determine w...
Topics
- Base Sequence
- Blotting, Northern
- Cell Line
- Cross-Linking Reagents
- DNA
- Insulin
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Phosphorylation
- Protein Precursors
- Receptor, Insulin