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Article

S-adenosyl-L-methionine is required for DNA cleavage by type III restriction enzymes.

Journal of molecular biology - 29 Jun 2001

Bist P, Sistla S, Krishnamurthy V, Acharya A, Chandrakala B, Rao D N

Abstract excerpt

The requirement of S-adenosyl-L-methionine (AdoMet) in the cleavage reaction carried out by type III restriction-modification enzymes has been investigated. We show that DNA restriction by EcoPI restriction enzyme does not take place in the absence of exogenously added AdoMet. Interestingly, the closely related EcoP15I enzyme has endogenously bound AdoMet and therefore does not require the addition of the...

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Identifiers

PMID: 11419939
DOI: 10.1006/jmbi.2001.4744

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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