Article
Electrophoretic and spectral characterization of wild type and mutant adenovirus protease.
The Journal of biological chemistry - 6 Oct 1995
Keyvani-Amineh H, Diouri M, Guillemette J G, Weber J M
Abstract excerpt
The P137L mutation in the adenovirus type 2 protease results in a temperature-sensitive protein-trafficking phenotype expressed during infection but not in vitro. Homology-derived secondary structure prediction placed the mutation within an externally disposed loop. Circular dichroism and urea gradient gel electrophoresis suggested that, unlike other thiol proteases, the Ad2 protease is comprised of a single...
Topics
- Adenoviruses, Human
- Amino Acid Sequence
- Circular Dichroism
- Electrophoresis
- Endopeptidases
- Enzyme Stability
- Molecular Sequence Data
- Molecular Structure
- Phenotype
- Point Mutation
- Protein Structure, Secondary