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Article

(77)Se enrichment of proteins expands the biological NMR toolbox.

Journal of molecular biology - 23 Jan 2013

Schaefer Stephanie A, Dong Ming, Rubenstein Renee P, Wilkie Wayne A, Bahnson Brian J, Thorpe Colin, Rozovsky Sharon

Abstract excerpt

Sulfur, a key contributor to biological reactivity, is not amendable to investigations by biological NMR spectroscopy. To utilize selenium as a surrogate, we have developed a generally applicable (77)Se isotopic enrichment method for heterologous proteins expressed in Escherichia coli. We demonstrate (77)Se NMR spectroscopy of multiple selenocysteine and selenomethionine residues in the sulfhydryl oxidase...

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Source

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Identifiers

PMID: 23159557
DOI: 10.1016/j.jmb.2012.11.011
PMCID: PMC3540199

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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