Article
Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR.
The journal of physical chemistry. B - 13 Aug 2015
Voinov Maxim A, Good Daryl B, Ward Meaghan E, Milikisiyants Sergey, Marek Antonin, Caporini Marc A, Rosay Melanie, Munro Rachel A, Ljumovic Milena, Brown Leonid S, Ladizhansky Vladimir, Smirnov Alex I
Abstract excerpt
Dynamic nuclear polarization (DNP) enhances the signal in solid-state NMR of proteins by transferring polarization from electronic spins to the nuclear spins of interest. Typically, both the protein and an exogenous source of electronic spins, such as a biradical, are either codissolved or suspended and then frozen in a glycerol/water glassy matrix to achieve a homogeneous distribution. While the use of such a...
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