Article
Integrative binding sites within intracellular termini of TRPV1 receptor.
PloS one - 1 Jan 2012
Grycova Lenka, Holendova Blanka, Bumba Ladislav, Bily Jan, Jirku Michaela, Lansky Zdenek, Teisinger Jan
Abstract excerpt
TRPV1 is a nonselective cation channel that integrates wide range of painful stimuli. It has been shown that its activity could be modulated by intracellular ligands PIP2 or calmodulin (CaM). The detailed localization and description of PIP2 interaction sites remain unclear. Here, we used synthesized peptides and purified fusion proteins of intracellular regions of TRPV1 expressed in E.coli in combination with...
Topics
- Animals
- Ankyrins
- Binding Sites
- Calmodulin
- Ligands
- Liposomes
- Molecular Docking Simulation
- Mutation
- Phosphatidylinositol Phosphates
- Protein Binding
- Protein Conformation
- Protein Interaction Domains and Motifs
- Rats
- Recombinant Fusion Proteins