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Optimized protocols for improving the likelihood of cloning recombinant progeny from Plasmodium yoelii genetic crosses.

Experimental parasitology - 1 Jan 2013

Qi Yanwei, Zhu Feng, Li Jian, Fu Yong, Pattaradilokrat Sittiporn, Hong Linxian, Liu Shengfa, Huang Fusheng, Xu Wenyue, Su Xin-Zhuan

Abstract excerpt

Genetic cross is a powerful tool for studying malaria genes contributing to drug resistance, parasite development, and pathogenesis. Cloning and identification of recombinant progeny (RP) is laborious and expensive, especially when a large proportion of progeny derived from self-fertilization are present in the uncloned progeny of a genetic cross. Since the frequency of cross-fertilization affects the number of...

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Identifiers

PMID: 23116600
DOI: 10.1016/j.exppara.2012.10.011
PMCID: PMC3530016

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