Article
Dynamics of intracellular clathrin/AP1- and clathrin/AP3-containing carriers.
Cell reports - 29 Nov 2012
Kural Comert, Tacheva-Grigorova Silvia K, Boulant Steeve, Cocucci Emanuele, Baust Thorsten, Duarte Delfim, Kirchhausen Tom
Abstract excerpt
Clathrin/AP1- and clathrin/AP3-coated vesicular carriers originate from endosomes and the trans-Golgi network. Here, we report the real-time visualization of these structures in living cells reliably tracked by rapid, three-dimensional imaging with the use of a spinning-disk confocal microscope. We imaged relatively sparse, diffraction-limited, fluorescent objects containing chimeric fluorescent protein (clathrin...
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