Article
Trafficking of yellow-fluorescent-protein-tagged mu1 subunit of clathrin adaptor AP-1 complex in living cells.
Traffic (Copenhagen, Denmark) - 1 May 2001
Huang F, Nesterov A, Carter R E, Sorkin A
Abstract excerpt
Clathrin adaptor protein AP-1 complex is thought to function in forming clathrin-coated vesicles at the trans-Golgi network (TGN) and mediating transport of cargo between the TGN and endosomes. To study trafficking of AP-1 in living cells, yellow fluorescent protein (YFP) was inserted in the middle of mu1 A subunit of AP-1. When expressed in a tetracycline-dependent manner in HeLa cells, YFP-mu1 was efficiently...
Topics
- Bacterial Proteins
- Fluorescent Antibody Technique
- Fluorescent Dyes
- Golgi Apparatus
- HeLa Cells
- Humans
- Indicators and Reagents
- Luminescent Proteins
- Mutation
- Plasmids
- Point Mutation