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Mutations in the substrate entrance region of β-glucosidase from Trichoderma reesei improve enzyme activity and thermostability.

Protein engineering, design & selection : PEDS - 1 Nov 2012

Lee Hsiao-Lin, Chang Chih-Kang, Jeng Wen-Yih, Wang Andrew H-J, Liang Po-Huang

Abstract excerpt

β-Glucosidase (EC 3.2.1.21) plays an essential role in biofuel production since it can cleave β-1,4-glycosidic bond to convert cellobiose into fermentable glucose. Based on the structure of Trichoderma reesei β-glucosidase 2 (TrBgl2) we solved, the amino acids in the outer channel of active site were mutated in this study. Mutants P172L and P172L/F250A showed the most enhanced k(cat)/K(m) and k(cat) values by...

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PMID: 23077275
DOI: 10.1093/protein/gzs073

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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