Article
Engineering the exo-loop of Trichoderma reesei cellobiohydrolase, Cel7A. A comparison with Phanerochaete chrysosporium Cel7D.
Journal of molecular biology - 31 Oct 2003
von Ossowski Ingemar, Ståhlberg Jerry, Koivula Anu, Piens Kathleen, Becker Dieter, Boer Harry, Harle Raija, Harris Mark, Divne Christina, Mahdi Sabah, Zhao Yongxin, Driguez Hugues, Claeyssens Marc, Sinnott Michael L, Teeri Tuula T
Abstract excerpt
The exo-loop of Trichoderma reesei cellobiohydrolase Cel7A forms the roof of the active site tunnel at the catalytic centre. Mutants were designed to study the role of this loop in crystalline cellulose degradation. A hydrogen bond to substrate made by a tyrosine at the tip of the loop was removed by the Y247F mutation. The mobility of the loop was reduced by introducing a new disulphide bridge in the mutant...
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