Article
From in silico to in vitro: modelling and production of Trichoderma reesei endoglucanase 1 and its mutant in Pichia pastoris.
Journal of biotechnology - 31 May 2012
Bayram Akcapinar Gunseli, Gul Ozgur, Sezerman Ugur O
Abstract excerpt
In this study, a major cellulase, namely endoglucanase 1 (EGI) from Trichoderma reesei was mutated by the introduction of four different lysine and glycine rich loops to create a hotspot for directed crosslinking of EGI away from the active site. The impact of the inserted loops on the stability of the enzyme was analyzed using molecular dynamics (MD) and the effect on the active site was studied using molecular...
Topics
- Bioreactors
- Carboxymethylcellulose Sodium
- Cellulase
- Computer Simulation
- Enzyme Stability
- Fungal Proteins
- Hydrogen-Ion Concentration
- Hydrolysis
- Molecular Dynamics Simulation
- Mutagenesis, Site-Directed
- Mutation
- Pichia
- Protein Engineering
- Recombinant Proteins
- Temperature
- Trichoderma