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Article

Engineering endoglucanase II from Trichoderma reesei to improve the catalytic efficiency at a higher pH optimum.

Journal of biotechnology - 1 Jun 2008

Qin Yuqi, Wei Xiaomin, Song Xin, Qu Yinbo

Abstract excerpt

The catalytic efficiency and pH optimum of Trichoderma reesei endo-beta-1,4-glucanase II were improved by protein engineering. We subjected residue 342 to saturation mutagenesis, and further changed the enzyme by random mutagenesis and two rounds of DNA shuffling. Enzyme variants were purified and characterized. Variant N342V exhibited an optimal activity at pH 5.8, corresponding to a basic shift of 1 pH unit...

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Source

Identifiers

PMID: 18468710
DOI: 10.1016/j.jbiotec.2008.03.016

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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