Article
Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16.
Current genetics - 1 May 2017
Tsai Yung-Yu, Ohashi Takao, Kanazawa Takenori, Polburee Pirapan, Misaki Ryo, Limtong Savitree, Fujiyama Kazuhito
Abstract excerpt
Rhodosporidium toruloides DMKU3-TK16 (TK16), a basidiomycetous yeast isolated in Thailand, can produce a large amount of oil corresponding to approximately 70 % of its dry cell weight. However, lack of a sufficient and efficient transformation method makes further genetic manipulation of this organism difficult. We here developed a new transformation system for R. toruloides using a lithium acetate method with...
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