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Article

Identification of novel genes in the carotenogenic and oleaginous yeast Rhodotorula toruloides through genome-wide insertional mutagenesis.

BMC microbiology - 21 Feb 2018

Liu Yanbin, Koh Chong Mei John, Yap Sihui Amy, Du Minge, Hlaing Mya Myintzu, Ji Lianghui

Abstract excerpt

BACKGROUND: Rhodotorula toruloides is an outstanding producer of lipids and carotenoids. Currently, information on the key metabolic pathways and their molecular basis of regulation remains scarce, severely limiting efforts to engineer it as an industrial host. RESULTS: We have adapted Agrobacterium tumefaciens-mediated transformation (ATMT) as a gene-tagging tool for the identification of novel genes in R....

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Identifiers

PMID: 29466942
DOI: 10.1186/s12866-018-1151-6
PMCID: PMC5822628

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