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Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the Δalg3 knockout mutant.

Glycobiology - 1 Feb 2013

Berends Elsa, Lehle Ludwig, Henquet Maurice, Hesselink Thamara, Wösten Han A B, Lugones Luis G, Bosch Dirk

Abstract excerpt

Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man(5)GlcNAc(2)-PP-Dol resulting in the formation of Man(6)GlcNAc(2)-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc(3)Man(9)GlcNAc(2) for N-glycosylation of proteins. Here, we identified the alg3 gene of the mushroom-forming fungus Schizophyllum commune by homology search. Its function was...

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PMID: 22997240
DOI: 10.1093/glycob/cws135

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