Article
Schizosaccharomyces pombe gmd3(+)/alg11(+) is a functional homologue of Saccharomyces cerevisiae ALG11 which is involved in N-linked oligosaccharide synthesis.
Yeast (Chichester, England) - 1 Oct 2000
Umeda K, Yoko-o T, Nakayama K, Suzuki T, Jigami Y
Abstract excerpt
The oligosaccharide of glycoproteins in the fission yeast Schizosaccharomyces pombe is unique in containing galactose. We isolated four mutants that had reduced amounts of galactose residues on their cell surface glycoproteins by fluorescence-activated cell sorter. The isolated four recessive mutants, gmd1 to gmd4, showed a defect in glycosylation of acid phosphatase, a cell surface glycoprotein. In gmd3 mutant...
Topics
- Acid Phosphatase
- Amino Acid Sequence
- Carbohydrate Conformation
- Cell Membrane
- Cloning, Molecular
- Fungal Proteins
- Galactose
- Genes, Fungal
- Genetic Complementation Test
- Glycosylation
- Mannans
- Molecular Sequence Data
- Mutation