Article
Prenatal diagnosis of beta-thalassemic mutations in Chinese by multiple restriction fragment-single strand conformation polymorphism analysis.
Proceedings of the National Science Council, Republic of China. Part B, Life sciences - 1 Jul 1994
Lee H H, Chang J G, Chen R T, Yang M L, Choo K B
Abstract excerpt
A modified approach of polymerase chain reaction (PCR)-based single strand conformation polymorphism (SSCP) has been developed for prenatal diagnosis of beta-thalassemic mutations. In this method, a single PCR product (1,350-bp) is first generated, which is then digested by restriction enzymes (BbsI, BamHI, DraI) to generate multiple shorter restriction fragments for electrophoretic analysis in an SSCP gel. The...
Topics
- Base Sequence
- China
- Chorionic Villi Sampling
- Female
- Fetal Diseases
- Globins
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction