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Article

Next-generation ion torrent sequencing of drug resistance mutations in Mycobacterium tuberculosis strains.

Journal of clinical microbiology - 1 Dec 2012

Daum Luke T, Rodriguez John D, Worthy Sue A, Ismail Nazir A, Omar Shaheed V, Dreyer Andries W, Fourie P Bernard, Hoosen Anwar A, Chambers James P, Fischer Gerald W

Abstract excerpt

A novel protocol for full-length Mycobacterium tuberculosis gene analysis of first- and second-line drug resistance was developed using the Ion Torrent Personal Genome Machine (PGM). Five genes-rpoB (rifampin), katG (isoniazid), pncA (pyrazinamide), gyrA (ofloxacin/fluoroquinolone), and rrs (aminoglycosides)-were amplified and sequenced, and results were compared to those obtained by genotypic Hain line probe...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 22972833
DOI: 10.1128/JCM.01893-12
PMCID: PMC3502959

Genes

LPA

Variants

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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