Article
Introduction of a tryptophan side chain into subsite +1 enhances transglycosylation activity of a GH-18 chitinase from Arabidopsis thaliana, AtChiC.
Glycobiology - 1 Jan 2013
Umemoto Naoyuki, Ohnuma Takayuki, Mizuhara Mamiko, Sato Hirokazu, Skriver Karen, Fukamizo Tamo
Abstract excerpt
A tryptophan side chain was introduced into subsite +1 of family GH-18 (class V) chitinases from Nicotiana tabacum and Arabidopsis thaliana (NtChiV and AtChiC, respectively) by the mutation of a glycine residue to tryptophan (G74W-NtChiV and G75W-AtChiC). The specific activity toward glycol chitin of the two mutant enzymes was 70-71% of that of the wild type. Using chitin oligosaccharides, (GlcNAc)(n) (n = 4, 5...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.