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Article

Role of tryptophan residues in a class V chitinase from Nicotiana tabacum.

Bioscience, biotechnology, and biochemistry - 1 Jan 2012

Umemoto Naoyuki, Ohnuma Takayuki, Urpilainen Henri, Yamamoto Takanori, Numata Tomoyuki, Fukamizo Tamo

Abstract excerpt

Tryptophan residues located in the substrate-binding cleft of a class V chitinase from Nicotiana tabacum (NtChiV) were mutated to alanine and phenylalanine (W190F, W326F, W190F/W326F, W190A, W326A, and W190A/W326A), and the mutant enzymes were characterized to define the role of the tryptophans....

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Source

Identifiers

PMID: 22484953
DOI: 10.1271/bbb.110914

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-22484953-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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