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Auxiliary active site mutations enhance the glycosynthase activity of a GH18 chitinase for polymerization of chitooligosaccharides.

Carbohydrate polymers - 15 Jan 2021

Alsina Cristina, Sancho-Vaello Enea, Aranda-Martínez Almudena, Faijes Magda, Planas Antoni

Abstract excerpt

Depolymerization of chitin results in chitooligosaccharides (COS) that induce immunostimulatory effects and disease protective responses and have many potential applications in agriculture and medicine. Isolation of bioactive COS with degree of polymerization (DP) larger than six from chitin hydrolyzates is hampered by their water insolubility. Enzymatic synthesis by exploiting the transglycosylation activity of...

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PMID: 33183587
DOI: 10.1016/j.carbpol.2020.117121

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