Article
Crystal structure and mode of action of a class V chitinase from Nicotiana tabacum.
Plant molecular biology - 1 Feb 2011
Ohnuma Takayuki, Numata Tomoyuki, Osawa Takuo, Mizuhara Mamiko, Vårum Kjell M, Fukamizo Tamo
Abstract excerpt
A class V chitinase from Nicotiana tabacum (NtChiV) with amino acid sequence similar to that of Serratia marcescens chitinase B (SmChiB) was expressed in E. coli and purified to homogeneity. When N-acetylglucosamine oligosaccharides [(NAG)(n)] were hydrolyzed by the purified NtChiV, the second glycosidic linkage from the non-reducing end was predominantly hydrolyzed in a manner similar to that of SmChiB. NtChiV...
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