A Naturally Occurring Mutation Within the Probe-Binding Region Compromises a Molecular-Based West Nile Virus Surveillance Assay for Mosquito Pools (Diptera: Culicidae)

Abstract excerpt

A naturally occurring mutation was detected within the probe binding region targeting the envelope gene sequence of West Nile virus used in real-time polymerase chain reaction assays to test mosquito pools and other samples. A single C-->T transition 6nt from the 5' end of the 16mer in the envelope gene probe-binding region at genomic position 1,194 reduced assay sensitivity. The mutation first was detected in...

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