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Article

A β-(1,2)-glycosynthase and an attempted selection method for the directed evolution of glycosynthases.

Biochemistry - 29 Nov 2011

Jakeman David L, Sadeghi-Khomami Ali

Abstract excerpt

Understanding how enzymes mediate catalysis is a key to their reprogramming for biotechnological applications. The family 3 retaining glycosidase postulated to be involved in erythromycin self-resistance was cloned, recombinantly expressed in Escherichia coli, purified, and characterized. Bioinformatics analysis allowed the identification of the acid/base and nucleophile residues, and mutation of these residues...

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Identifiers

PMID: 22035228
DOI: 10.1021/bi201438q

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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