Article
DNA restriction is a barrier to natural transformation in Pseudomonas stutzeri JM300.
Microbiology (Reading, England) - 1 Apr 2003
Berndt Cecilia, Meier Petra, Wackernagel Wilfried
Abstract excerpt
Natural transformation is a mechanism for intra- and interspecific transfer of chromosomal DNA in Pseudomonas stutzeri. During this process a single strand derived from duplex DNA is transported into the cytoplasm and recombined with resident DNA. By electroporation, which introduces duplex DNA into cells, 100-fold lower transformation frequencies of P. stutzeri JM300 were observed with shuttle vector or...
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