Article
Construction of recA mutants of Acholeplasma laidlawii by insertional inactivation with a homologous DNA fragment.
Plasmid - 1 Nov 1992
Dybvig K, Woodard A
Abstract excerpt
Mycoplasmas (class Mollicutes) are wall-less prokaryotes phylogenetically related to gram-positive bacteria. This study describes the construction of recA mutants of the mycoplasma Acholeplasma laidlawii. An internal fragment of the recA gene from A. laidlawii was cloned into a plasmid that does not replicate in this organism. When this plasmid construct was used to transform A. laidlawii, it inserted into the...
Topics
- Acholeplasma laidlawii
- Base Sequence
- DNA Repair
- DNA, Bacterial
- Genes, Bacterial
- Genetic Vectors
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype
- Plasmids
- Rec A Recombinases