The C-terminal domain of eukaryotic initiation factor 5 promotes start codon recognition by its dynamic interplay with eIF1 and eIF2β.

doi:10.1016/j.celrep.2012.04.007

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Article

The C-terminal domain of eukaryotic initiation factor 5 promotes start codon recognition by its dynamic interplay with eIF1 and eIF2β.

Cell reports - 28 Jun 2012

Luna Rafael E, Arthanari Haribabu, Hiraishi Hiroyuki, Nanda Jagpreet, Martin-Marcos Pilar, Markus Michelle A, Akabayov Barak, Milbradt Alexander G, Luna Lunet E, Seo Hee-Chan, Hyberts Sven G, Fahmy Amr, Reibarkh Mikhail, Miles David, Hagner Patrick R, O'Day Elizabeth M, Yi Tingfang, Marintchev Assen, Hinnebusch Alan G, Lorsch Jon R, Asano Katsura, Wagner Gerhard

Abstract excerpt

Recognition of the proper start codon on mRNAs is essential for protein synthesis, which requires scanning and involves eukaryotic initiation factors (eIFs) eIF1, eIF1A, eIF2, and eIF5. The carboxyl terminal domain (CTD) of eIF5 stimulates 43S preinitiation complex (PIC) assembly; however, its precise role in scanning and start codon selection has remained unknown. Using nuclear magnetic resonance (NMR)...

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PMID: 22813744
DOI: 10.1016/j.celrep.2012.04.007
PMCID: PMC3401385

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