Article
Roles of heterotypic CCN2/CTGF-CCN3/NOV and homotypic CCN2-CCN2 interactions in expression of the differentiated phenotype of chondrocytes.
The FEBS journal - 1 Oct 2012
Hoshijima Mitsuhiro, Hattori Takako, Aoyama Eriko, Nishida Takashi, Yamashiro Takashi, Takigawa Masaharu
Abstract excerpt
To identify proteins that regulate CCN2 activity, we carried out GAL4-based yeast two-hybrid screening with a cDNA library derived from a chondrocytic cell line, HCS-2/8. CCN2/CTGF and CCN3/NOV polypeptides were picked up as CCN2-binding proteins, and CCN2–CCN2 and CCN2–CCN3 binding domains were identified. Direct binding between CCN2 and CCN3 was confirmed by coimmunoprecipitation in vitro and in vivo and...
Topics
- Aggrecans
- Animals
- Blotting, Western
- COS Cells
- Cell Differentiation
- Cells, Cultured
- Chlorocebus aethiops
- Chondrocytes
- Collagen Type II
- Connective Tissue Growth Factor
- Fluorescence
- Fluorescent Antibody Technique, Indirect
- Humans