Article
CENP-E--dependent BubR1 autophosphorylation enhances chromosome alignment and the mitotic checkpoint.
The Journal of cell biology - 23 Jul 2012
Guo Yige, Kim Christine, Ahmad Sana, Zhang Jiayin, Mao Yinghui
Abstract excerpt
How the state of spindle microtubule capture at the kinetochore is translated into mitotic checkpoint signaling remains largely unknown. In this paper, we demonstrate that the kinetochore-associated mitotic kinase BubR1 phosphorylates itself in human cells and that this autophosphorylation is dependent on its binding partner, the kinetochore motor CENP-E. This CENP-E-dependent BubR1 autophosphorylation at...
Topics
- Aurora Kinase B
- Aurora Kinases
- Cell Line
- Chromosomal Proteins, Non-Histone
- Chromosomes
- Cytoskeletal Proteins
- Humans
- Kinetochores
- M Phase Cell Cycle Checkpoints
- Metaphase
- Mutation