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Article

A comprehensive procedural approach to genotyping KRAS and BRAF from paraffin embedded tissues for diagnostic purposes.

In vivo (Athens, Greece) - 1 Jan 2000

Palmirotta Raffaele, Ludovici Giorgia, De Marchis Maria Laura, Leone Barbara, Formica Vincenzo, Ettorre Giuseppe Maria, Cavaliere Francesco, Della-Morte David, Ferroni Patrizia, Roselli Mario, Guadagni Fiorella

Abstract excerpt

BACKGROUND: Mutations in the Kirsten Ras 1 (KRAS) and V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) genes may be predictive of response to drugs directly linked to the Epidermal Growth Factor Receptor (EGFR) signaling pathway. MATERIALS AND METHODS: A total of 230 samples from patients with metastatic colorectal cancer were analyzed for KRAS exon 1 and 2 and for BRAF exon 15 mutations. DNA from...

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Identifiers

PMID: 22773565

Genes

BRAF
KRAS
EGFR

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