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Article

Engineering disulfide bonds in Selenomonas ruminantium β-xylosidase by experimental and computational methods.

International journal of biological macromolecules - 1 Feb 2017

Dehnavi Ehsan, Fathi-Roudsari Mehrnoosh, Mirzaie Sako, Arab Seyed Shahriar, Ranaei Siadat Seyed Omid, Khajeh Khosro

Abstract excerpt

Homotetrameric β-xylosidase from Selenomonas ruminantium (SXA) is one of the most efficient enzymes known for the hydrolysis of cell wall hemicellulose. SXA shows a rapid rate of activity loss at temperatures above 50°C. In this study, we have introduced two inter-subunit disulfide bridges with one point mutation. Lys237 was chosen to be replaced with cysteine since it interacts with the same residue in the...

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Identifiers

PMID: 27818293
DOI: 10.1016/j.ijbiomac.2016.10.104

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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