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Article

Direct genotyping of C3435T single nucleotide polymorphism in unamplified human MDR1 gene using a surface plasmon resonance imaging DNA sensor.

Analytical and bioanalytical chemistry - 1 May 2015

Mariani Stefano, Scarano Simona, Carrai Maura, Barale Roberto, Minunni Maria

Abstract excerpt

Optical genotyping of C3435T single nucleotide polymorphisms (SNPs) in unamplified human multidrug resistance (MDR1) gene was here performed by a surface plasmon resonance imaging (SPRi) dual-targeting DNA assay, allowing its selective detection down to 0.18 fM of the whole genomic DNA. The result was achieved by the combination of the rational selection of the DNA probe and an optimized sample pretreatment...

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PMID: 25619982
DOI: 10.1007/s00216-014-8424-1

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