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Article

Are free radicals involved in IspH catalysis? An EPR and crystallographic investigation.

Journal of the American Chemical Society - 11 Jul 2012

Wang Weixue, Wang Ke, Span Ingrid, Jauch Johann, Bacher Adelbert, Groll Michael, Oldfield Eric

Abstract excerpt

The [4Fe-4S] protein IspH in the methylerythritol phosphate isoprenoid biosynthesis pathway is an important anti-infective drug target, but its mechanism of action is still the subject of debate. Here, by using electron paramagnetic resonance (EPR) spectroscopy and (2)H, (17)O, and (57)Fe isotopic labeling, we have characterized and assigned two key reaction intermediates in IspH catalysis. The results are...

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PMID: 22687151
DOI: 10.1021/ja303445z
PMCID: PMC3394908

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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