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Article

Accurate Estimation of Fungal Diversity and Abundance through Improved Lineage-Specific Primers Optimized for Illumina Amplicon Sequencing.

Applied and environmental microbiology - 15 Dec 2016

Taylor D Lee, Walters William A, Lennon Niall J, Bochicchio James, Krohn Andrew, Caporaso J Gregory, Pennanen Taina

Abstract excerpt

While high-throughput sequencing methods are revolutionizing fungal ecology, recovering accurate estimates of species richness and abundance has proven elusive. We sought to design internal transcribed spacer (ITS) primers and an Illumina protocol that would maximize coverage of the kingdom Fungi while minimizing nontarget eukaryotes. We inspected alignments of the 5.8S and large subunit (LSU) ribosomal genes and...

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Identifiers

PMID: 27736792
DOI: 10.1128/AEM.02576-16
PMCID: PMC5118932

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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