Article
Computational methods for high-throughput comparative analyses of natural microbial communities.
Methods in enzymology - 1 Jan 2013
Preheim Sarah P, Perrotta Allison R, Friedman Jonathan, Smilie Chris, Brito Ilana, Smith Mark B, Alm Eric
Abstract excerpt
One of the most widely employed methods in metagenomics is the amplification and sequencing of the highly conserved ribosomal RNA (rRNA) genes from organisms in complex microbial communities. rRNA surveys, typically using the 16S rRNA gene for prokaryotic identification, provide information about...
Topics
- Classification
- Computational Biology
- Genetic Variation
- High-Throughput Nucleotide Sequencing
- Humans
- Metagenomics
- Microbial Consortia
- Phylogeny
- RNA, Ribosomal, 16S
- Sequence Analysis, DNA