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Article

Soil bacterial diversity screening using single 16S rRNA gene V regions coupled with multi-million read generating sequencing technologies.

PloS one - 1 Jan 2012

Vasileiadis Sotirios, Puglisi Edoardo, Arena Maria, Cappa Fabrizio, Cocconcelli Pier S, Trevisan Marco

Abstract excerpt

The novel multi-million read generating sequencing technologies are very promising for resolving the immense soil 16S rRNA gene bacterial diversity. Yet they have a limited maximum sequence length screening ability, restricting studies in screening DNA stretches of single 16S rRNA gene hypervariable (V) regions. The aim of the present study was to assess the effects of properties of four consecutive V regions...

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Identifiers

PMID: 22880076
DOI: 10.1371/journal.pone.0042671
PMCID: PMC3412817

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Source receipt: pubmed, retrieved 7 Sept 2026.

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