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Article

A method for determining zygosity of transgenic zebrafish by TaqMan real-time PCR.

Analytical biochemistry - 15 Sept 2005

Ji Wan, Zhou Wenli, Abruzzese Ron, Guo Wei, Blake Allan, Davis Scott, Davis Sara, Polejaeva Irina

Abstract excerpt

When producing a genetically modified organism, intended genes are often integrated into a target genome by random insertions. Subsequently, it is often desirable to know the gene copy number of the transgenic organism and the zygosity of its offspring. Because of the random insertions, the estimation can be made only by quantitative measurement of the genes. Even though TaqMan real-time PCR has been used in gene...

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PMID: 16061191
DOI: 10.1016/j.ab.2005.06.046

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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